How to Detect EGFP Enhanced Green Fluorescent Protein

Published: 2024-04-26
Views:

Enhanced Green Fluorescent Protein (EGFP)

Enhanced green fluorescent protein (EGFP) is a widely used variant of GFP. Compared with wild-type GFP, EGFP is brighter and matures rapidly at 37°C. EGFP is a powerful tool for the visualization of tagged proteins and transfected cells in molecular and biological research.

To detect EGFP fluorescence, the first step is to learn the excitation and emission wavelength of EGFP. Based on the spectrum of EGFP fluorescent protein, choose a fluorescence detector that has an appropriate excitation wavelength for EGFP. As shown in the following figure, EGFP can be excited by the 488 nm laser line and is optimally detected at 507 nm.

Figure below: excitation and emission wavelength of EGFP fluorescent protein

excitation and emission wavelength of EGFP fluorescent protein

Detect EGFP Fluorescent Protein Using GFPfinder-2101RB

GFPfinder-2101RB Fluorescence Flashlight is a powerful tool to detect GFP and EGFP fluorescent proteins in plants, leaves, seeds, roots, cells, and animals. Using GFPfinder-2101RB, you can detect GFP and EGFP fluorescence in seconds! 

Features of GFPfinder-2101RB Fluorescence Flashlight

● Wide applications: GFPfinder-2101RB Fluorescence Flashlight can be used to detect GFP and EGFP fluorescent proteins in plants, leaves, seeds, roots, cells, and animals (e.g. mice, zebra fish).

● Intense excitation light: Equipped with 12 watt high-power LEDs, GFPfinder-2101RB Fluorescence Flashlight can detect weak fluorescence in the dark!

● Portability: Powered by a rechargeable battery, you can use GFPfinder-2101RB Fluorescence Flashlight in the field and detect EGFP fluroescence in living plants.

● Efficiency: No pre-heating is required. Turn on the flashlight, and detect EGFP fluorescence in seconds with the naked eye!


Figure below: EGFP fluorescence in leaves detected by GFPfinder-2101RB Fluorescence Flashlight

EGFP fluorescence in leaves

Figure below: GFPfinder-2101RB designed for the detection of GFP and EGFP fluorescence

EGFP fluorescence flashlight


Tags: Fluorescent Protein, Fluorescence Flashlight, Fluorescent Protein Detector,
Related News
Read More>>
Inspection of PCB Conformal Coating using UV Light Inspection of PCB Conformal Coating using UV Light
09.09.2024
Conformal coating is a coating used to protect printed circuit boards (PCBs) and
Detection of SGFP2 Green Fluorescent Protein Detection of SGFP2 Green Fluorescent Protein
08.16.2024
The SGFP2 fluorescent protein is a green fluorescent protein variant, published in 2007, derived fro...
Handheld UV Lamp 254nm/365nm Handheld UV Lamp 254nm/365nm
06.11.2024
Handheld UV Lamp 254nm/365nmThe XEPU Handheld UV Lamp 254nm/365nm (Model number:
Black Light UV Flashlight XEPU-1530B for NDT Black Light UV Flashlight XEPU-1530B for NDT
06.06.2024
The XEPU-1530B Black Light UV Flashlight features a powerful UV-A (365 nm) LED light source and a lo...

Leave Your Message